interferon-gamma gene polymorphism +874 (a/t) in chinese children with henoch-schonlein purpura.

نویسندگان

hui xu department of clinical laboratory, children's hospital of zhejiang university school of medicine, hangzhou, p.r. china.

wei li department of clinical laboratory, children's hospital of zhejiang university school of medicine, hangzhou, p.r. china.

haidong fu department of nephrology, children's hospital of zhejiang university school of medicine, hangzhou, p.r. china.

guizheng jiang department of clinical laboratory, children's hospital of zhejiang university school of medicine, hangzhou, p.r. china.

چکیده

the aim of this study was to investigate the possible influence of interferon-gamma (ifn- γ) gene polymorphism +874 (a/t) (rs2430561) in the susceptibility and renal complications of patients with henoch-schonlein purpura (hsp). we also studied the effects of ifn-γ allelic variation on serum levels of pro-and anti-inflammatory cytokines in hsp patients. the  study  population  comprised  97  patients  suffering  from  hsp  and  97  control participants. patients and controls  were genotyped for a single nucleotide polymorphism +874 (a/t) in the first intron of the ifn-γ gene by the taqman pcr method. frequencies of individuals with ifn-γ  +874  aa, at and tt genotypes were 77.3%,21.6% and 1% in hsp patients and 79.4%, 17.5% and 3.1% in controls, respectively. the frequency of the aa genotype in hsp patients with nephritis was slightly higher (83.3%) than in hsp patients without nephritis (73.8%). the allele a occurred more commonly in hsp patients with nephritis (92%) than in hsp patients without nephritis (86%), but these differences were not statistically significant (p= 0.469 and p= 0.244, respectively). in addition, significant difference in serum il-10 levels between ifn-γ +874 different genotype groups was found. our  results do not  support  a role for ifn-γ  gene polymorphism +874  (a/t) in the susceptibility to hsp and allelic variation at ifn-γ +874 locus had no effect on serum levels of cytokines in patients with hsp except for il-10.

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عنوان ژورنال:
iranian journal of allergy, asthma and immunology

جلد ۱۳، شماره ۳، صفحات ۱۸۴-۱۸۹

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